TEAC: Trolox Equivalent

Antioxidant Capacity. Physical activity and dietary intake Subjects were instructed to maintain their normal physical activity throughout the study period, with the exception of refraining from strenuous physical activity during the 24 hours prior to each test day and during the 48 hours following each test day. They were also given specific instructions regarding abstinence from LY2874455 alcohol, medication, and dietary supplement consumption during the 24 hours immediately before the test days and during the 48 hours following each test day. Dietary intake was to be maintained as usual through the study period, with the exception of reporting to the lab in a fasted state on each of the two test days (no food, RAD001 research buy caffeine, or calorie containing beverages allowed after midnight). No food records were maintained in this study, which may be considered a limitation of this work. Exercise test days On each of the two exercise test days, subjects STA-9090 manufacturer reported to the lab in the morning following an overnight fast. However, subjects were instructed to consume water liberally up to the time they reported to the lab for testing. Adherence to study instructions was confirmed with all subjects on each day of testing by use of a dichotomous questionnaire. Specifically, on the day of testing, we used an in-lab questionnaire

asking subjects if they consumed any food since midnight the night before, or any alcohol, caffeine, or nutritional supplements during the prior 24 hours. We also used phone questionnaires during the study period asking subjects if they exercised since the last study Farnesyltransferase visit, used any vitamin and/or mineral supplements since the last study visit, or taken acetaminophen since the last study visit. For testing days, the time of day for each subject was matched for the subsequent test day. Following all baseline measurements and approximately 45 minutes prior to the

start of the knee extension exercise protocol, subjects were provided with a standardized breakfast consisting of a bagel, one tablespoon of low fat cream cheese, 8 ounces of orange juice, and water ad libitum. On the test days, subjects took their assigned MSM dose immediately prior to the standardized breakfast. For the exercise test, subjects performed a total of 18 sets of knee extension exercise using a plate-loaded machine (Key Fitness Products, LP; Garland, TX). Sets 1–15 were performed at a predetermined weight for 10 repetitions each, while sets 16–18 were performed to muscular failure. Specifically, subjects performed 5 sets of 10 repetitions at 30% 1-RM for a total of 50 repetitions, followed by a 3 minute rest. Subjects then performed 5 sets of 10 repetitions at 45% 1-RM for a total of 50 repetitions, followed by a 3 minute rest.