APEC_O1 strain was kindly provided by Lisa K. Nolan (Iowa State University, Ames, USA) and fim negative E. coli strain AAEC189 by Ulrich Dobrindt (Julius-Maximilians Pifithrin �� Universität Wuerzburg, Germany), respectively. This work was supported by the government of the People’s Republic of China, the Eltanexor cell line Sino-German Cooperation on Agricultural Science

and Technology and by grants from the Deutsche Forschungsgemeinschaft (WI 1436/5-3). Electronic supplementary material Additional file 1: Oligonucleotide primers used in this study. Names and nucleotide sequences of oligonucleotide primers used in this study. (DOC 51 KB) References 1. Kaper JB: Pathogenic Escherichia coli. Int J Med Microbiol 2005,295(6–7):355–356.PubMedCrossRef 2. Kim KS: Meningitis-Associated Escherichia coli. In Escherichia coli: Virulence Mechanisms of a Versatile Pathogen. www.selleckchem.com/products/AZD7762.html Edited by: Orlando MSD. Florida, USA: Academic Press;

2002:269–286. 3. Barnes HJ, Gross WB: Colibacillosis. In Diseases of poultry. 10th edition. Edited by: Gross WB. Ames: Iowa State University Press; 1999:131–141. 4. Dobrindt U: (Patho-)Genomics of Escherichia coli. Int J Med Microbiol 2005,295(6–7):357–371.PubMedCrossRef 5. Blondeau JM: Current issues in the management of urinary tract infections: extended-release ciprofloxacin as a novel treatment option. Drugs 2004,64(6):611–628.PubMedCrossRef 6. Ewers C, Janssen T, Wieler LH: [Avian pathogenic Escherichia coli (APEC)]. Berl Munch Tierarztl Wochenschr 2003,116(9–10):381–395.PubMed 7. Johnson TJ, Wannemuehler Y, Johnson SJ, Stell AL, Doetkott C, Johnson JR, Kim KS, Spanjaard L, Nolan LK: Comparison of extraintestinal pathogenic Escherichia coli strains from human and avian sources reveals a mixed subset representing potential zoonotic pathogens. Appl Environ Masitinib (AB1010) Microbiol 2008,74(22):7043–7050.PubMedCrossRef 8. Ewers C, Li G, Wilking H, Kiessling S, Alt K, Antão E-M, Laturnus C, Diehl I, Glodde S, Homeier

T, et al.: Avian pathogenic, uropathogenic, and newborn meningitis-causing Escherichia coli: How closely related are they? Int J Med Microbiol 2007,297(3):163–176.PubMedCrossRef 9. Moulin-Schouleur M, Schouler C, Tailliez P, Kao MR, Bree A, Germon P, Oswald E, Mainil J, Blanco M, Blanco J: Common virulence factors and genetic relationships between O18:K1:H7 Escherichia coli isolates of human and avian origin. J Clin Microbiol 2006,44(10):3484–3492.PubMedCrossRef 10. Li G, Laturnus C, Ewers C, Wieler LH: Identification of genes required for avian Escherichia coli septicemia by signature-tagged mutagenesis. Infect Immun 2005,73(5):2818–2827.PubMedCrossRef 11. Rouquet G, Porcheron G, Barra C, Reperant M, Chanteloup NK, Schouler C, Gilot P: A metabolic operon in extraintestinal pathogenic Escherichia coli promotes fitness under stressful conditions and invasion of eukaryotic cells. J Bacteriol 2009,191(13):4427–4440.PubMedCrossRef 12. Wells TJ, Tree JJ, Ulett GC, Schembri MA: Autotransporter proteins: novel targets at the bacterial cell surface.