Appl Environ Microbiol 1988,54(3):703–711.PubMed 18. Nüsslein K, Tiedje JM: Characterization of the dominant and rare members of a young Hawaiian soil bacterial community with small-subunit ribosomal DNA amplified from DNA fractionated on the basis of its guanine and cytosine composition. Appl Environ Microbiol 1998,64(4):1283–1289.PubMed 19. Holben WE, Feris KP, Kettunen A, Apajalahti JH: GC fractionation enhances microbial community diversity assessment and detection of minority populations of bacteria
by denaturing gradient gel electrophoresis. Appl Environ Microbiol 2004,70(4):2263–2270.CrossRefPubMed 20. Holben WE, Harris D: see more DNA-based monitoring of total bacterial community structure in environmental samples. Mol Ecol 1995,4(5):627–631.CrossRefPubMed 21. Kassinen A, Krogius-Kurikka L, Mäkivuokko H, Rinttilä Selleckchem C646 T, Paulin L, Corander J, Malinen E, Apajalahti J, Palva A: The fecal microbiota of irritable bowel syndrome
patients differs significantly from that of healthy subjects. Gastroenterology 2007,133(1):24–33.CrossRefPubMed 22. Galtier selleck N, Lobry JR: Relationships between genomic G+C content, RNA secondary structures, and optimal growth temperature in prokaryotes. J Mol Evol 1997,44(6):632–636.CrossRefPubMed 23. Good IJ: The population frequencies of species and the estimation of population parameters. Biometrika 1953, 40:237–264. 24. Schloss PD, Handelsman J: Introducing SONS, a tool for operational taxonomic unit-based comparisons of microbial community memberships and structures. Appl Environ Microbiol 2006,72(10):6773–6779.CrossRefPubMed 25. Wilson KH, Blitchington RB: Human colonic biota studied by ribosomal DNA sequence analysis. Appl Environ Microbiol 1996,62(7):2273–2278.PubMed 26. Suau A, Bonnet R, Sutren M, Godon JJ, Gibson GR, Collins MD, Doré J: Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Methane monooxygenase Microbiol 1999,65(11):4799–4807.PubMed 27. Bonnet R, Suau A, Doré J, Gibson
GR, Collins MD: Differences in rDNA libraries of faecal bacteria derived from 10- and 25-cycle PCRs. Int J Syst Evol Microbiol 2002,52(Pt 3):757–763.CrossRefPubMed 28. Eckburg PB, Bik EM, Bernstein CN, Purdom E, Dethlefsen L, Sargent M, Gill SR, Nelson KE, Relman DA: Diversity of the human intestinal microbial flora. Science 2005,308(5728):1635–1638.CrossRefPubMed 29. Li M, Wang B, Zhang M, Rantalainen M, Wang S, Zhou H, Zhang Y, Shen J, Pang X, Zhang M, Wei H, Chen Y, Lu H, Zuo J, Su M, Qiu Y, Jia W, Xiao C, Smith LM, Yang S, Holmes E, Tang H, Zhao G, Nicholson JK, Li L, Zhao L: Symbiotic gut microbes modulate human metabolic phenotypes. Proc Natl Acad Sci USA 2008,105(6):2117–2122.CrossRefPubMed 30. Hayashi H, Sakamoto M, Benno Y: Phylogenetic analysis of the human gut microbiota using 16S rDNA clone libraries and strictly anaerobic culture-based methods.