Approximately 20% of breast cancers overexpress HER2, caused by amplification of the erbB2 oncogene [11], [12], [13] and [14]. As a marker of aggressive disease,

Ceritinib research buy HER2 overexpression is an independent predictor of decreased recurrence-free survival, breast cancer-related survival, and overall survival [15] and [16]. The development of HER2-targeting therapy has revolutionized the treatment of HER2-positive breast cancer such that we may consider HER2 overexpression a positive predictor of improved outcome. Studies worldwide have identified the significant benefit of first-line trastuzumab therapy in conjunction with surgery and cytotoxic chemotherapy for treating HER2-positive breast carcinoma [17] and [18]. Thus, accurate HER2 testing

to ensure that the right patient receives the right treatment is now more critical than ever [19], [20] and [21]. Currently, we evaluate HER2 status mainly with immunohistochemistry (IHC) or fluorescence in situ hybridization (FISH); IHC analysis is usually used as the primary assay, and reflex FISH is performed for a specific subset of IHC results (e.g., 1+ or 2+); other laboratories primarily use FISH [22] and [23]. The 2013 ASCO/CAP (American Society of Clinical Oncology/College 5-FU purchase of American Pathologists) guideline defines HER2-positive breast carcinoma as tumors containing >10% of cells with complete and intense membrane staining Phloretin by IHC. FISH-positive

breast carcinoma is defined as average HER2 copy number ≥ 6.0 signals/cell or average HER2 copy number ≥ 4.0 signals/cell and HER2/chromosome 17 centromere (CEP17) ratio ≥ 2.0 [24]. In comparison, the 2007 ASCO/CAP guideline uses a cutoff value of HER2/CEP17 ratio > 2.2 to define HER2 overexpression [24], [25] and [26]. The 2013 criteria benefits many more patients in terms of the targeted drugs they may potentially receive, especially patients with chromosome 17 polysomy (polysomy 17) as identified by dual-probe FISH. In terms of HER2 gene assessment, it has been proven that CEP17 amplification causes misleading HER2 FISH results [27], [28], [29], [30] and [31], precluding anti-HER2-based therapy for some patients. In this study, we used the 2013 ASCO/CAP scoring criteria to evaluate HER2 amplification status in breast carcinoma with polysomy 17. The study involved 175 cases with primary invasive breast cancer. Samples were obtained after the patients had provided informed consent; the Nanjing Drum Tower Hospital Ethics Committee approved the study. The HER2 IHC was determined and we reviewed the HER2 status of the archived samples, and analyzed the tumors according to the 2007 and 2013 ASCO/CAP guidelines. Each tissue sample was fixed immediately in 10% neutral buffered formalin for 6–48 h, and then paraffin-embedded.