As a result, it is desirable to investigate the pumping effect of the solution with different concentrations. As reported by Tavares and McGuffin [23], the zeta potential varied linearly with the

logarithm of the ion concentration, meaning that the zeta potential decayed exponentially with Necrostatin-1 solubility dmso respect to the ion concentration. Thus, the relation between the EO flow rate and the ion concentration is an exponentially decay function under the influence of the electric field strength according to Equation 1. To examine the effect of the concentration dependency with respect to our device, the EO flow rates were measured at different ion concentrations when a GSK872 cost constant voltage of 3 V was applied, where the ion concentration refers

to the concentration of analytes in PBS learn more in this case. The ion concentrations were normalized by the standard PBS with a K2HPO4 concentration of 27.5 mM and a KH2PO4 concentration of 20.0 mM. After analyzing the fluorescent intensity of the acquired images using imaging software, the relation of EO flow rates versus different analyte concentrations was determined and is shown in Figure  6. The analytical relation between the EO flow rate and the ion concentration was determined and exhibited exponential decay characteristics. The resulting relation is v = 1.10583 + 15.7236 × e - 18.0505 ⋅ c , where v is the flow rate in the unit of picoliter per second and c represents the analyte concentration after normalization by standard

PBS. For a constant applied voltage, the higher the Exoribonuclease concentration, the lower the EO flow rate due to the decrease in zeta potential. After obtaining this relation, it is possible to estimate the flow rate of any diluted PBS driven by an applied voltage of 3 V. This method of investigating the effect of ion concentration on the EO flow rate is also applicable to other types of solution containing different analytes. Figure 6 The influence of ion concentration on the electroosmotic flow rate that exhibited an exponential function. The ion concentration was normalized by standard PBS with a K2HPO4 concentration of 27.5 mM and a KH2PO4 concentration of 20.0 mM. Program-controlled reaction in continuous flow Controlled chemical reaction is one of the potential applications of our nanofluidic device, and we employed the binding reaction between Fluo-4 and calcium chloride to demonstrate the feasibility of such application. Fluo-4 is a kind of chemical widely used in living cells as a calcium indicator. Its emitted fluorescent intensity was found to be linearly proportional to the calcium concentration for a particular range [24]. Here, pumping of calcium ions was controlled by LabVIEW which generates square waves with a fixed applied voltage of 3 V and different duty cycles. The EO flow rate of the calcium chloride from channel A to channel B was measured to be 1.