At the same time developed countries also can be benefited because of safety profile of the plant extracts and microbial strains agents. Key Word(s): 1. Anti-inflammatory; CDK phosphorylation 2. Plant extracts; 3. Tannic acid; 4. Wistar rats; Presenting Author: Ujjala Ghoshal Additional Authors: Sonali Khanduja, Priyannk Pant, Vikas Agarwal, Soniya Nityanand, RajKumar Sharma, UdayChand Ghoshal Corresponding Author:

Ujjala Ghoshal Affiliations: Sanjay Gandhi Postgraduate Institute of Medical Sciences Objective: Microsporidia, an opportunistic pathogen, is known to cause chronic diarrhoea in human immunodeficiency virus (HIV) infected patients. Other immunosuppressive states like renal transplantation

(RT) and haematological malignancy (HM) may also be at risk. However, data BI 6727 datasheet on prevalence and genetic characterization of microsporidia in RT and HM is scanty. Therefore, we aimed to study, a) Prevalence of microsporidia among HIV, RT and HM patients and healthy subjects, b) genetic characterization of microsporidia. Methods: 1910 stool samples from 730 immunocompromised patients and 170 healthy subjects were examined from January 2006 to March 2013 prospectively. The demographic and clinical details of the patients were recorded. Stool samples were examined for microsporidia by modified trichrome stain and PCR. Restriction fragment length polymorphism (RFLP) was done for identification of Enterocytozoon bieneusi, Encephalitozoon intestinalis, Enc. hellem, Enc. cuniculi. Results were confirmed by sequencing. Results: 29/730 (4%) immunocompromised patients (26/29, SB-3CT 89.7% male) and none of the healthy controls had microsporidia (29/730, 4% vs. 0/170,

0%; P < 0.05) either by PCR or microscopy. Intestinal microsporidiosis was comparable among patients with HIV, RT and HM (5/195, 2.5% vs. 22/387, 5.7% vs. 2/148, 1.4%; P = ns). Patients with microsporidia more often had diarrhoea than those without (24/342, 7% vs. 5/388, 1.3%; P < 0.05). Patients with microsporidia more often presented with chronic diarrhoea than acute diarrhoea (20/104, 19.2% vs. 4/238, 1.7%, P < 0.05). Other clinical features like fever, abdominal pain, chest pain, vomiting, presence of pets, type of drinking water was comparable among patients with and without microsporidia. E. bieneusi were detected in 28/29 (96.5%) patients and was confirmed by sequencing. Conclusion: Prevalence of Microsporidia in Immunocompromised patient is 4%, patients with chronic diarrhoea are at an increased risk. E. bieneusi is the most common species. Key Word(s): 1. Microsporidia; 2. Immunocompromised ; 3.