(C) 2009 Wiley Periodicals, Inc. J Appl Polym Sci 115: 2892-2897, 2010″
“Disorganized muscle family member (Dim-1) belongs to immunoglobulin superfamily, and is a structural protein localized to the region of the muscle cell membrane

around and between the dense bodies. Strong immunogenicity to host’s immune system was induced by Dim-1 from Ascaris suum, which indicated that Dim-1 could be a potential candidate for vaccine. The homologues of Dim-1 were also detected in nematodes Brugia malayi and Trichostrongylus colubriformis. However, information on the complete coding sequence and protection potential of this molecule in Haemonchus find more contortus is lacking. In this study, full length of Dim-1 cDNA was cloned using a rapid amplification of cDNA ends (RACE) strategy and the DNA vaccine encoding Dim-1 open reading frame (ORF) was tested for protection against experimental H. contortus infections in goats. Fifteen goats were allocated into three trial groups. The animals of Dim-1 group were vaccinated with the DNA vaccine encoding Dim-1 on day 0 and 14, and challenged with 5000 infective

H. contortus third larval stage (L3) on day 28. An unvaccinated positive control group was challenged with L3 at the same time. An unvaccinated negative control group was not challenged with L3. The results indicated Dim-1 DNA vaccines were transcribed at local injection sites and expressed in vivo post immunizations respectively. Following L3 challenge, the mean eggs per gram feces (EPG) and worm burdens of Dim-1 group were reduced by 45.7% and 51.1%, respectively. Significantly high levels of Selleckchem Entinostat serum IgG, Torin 2 ic50 serum IgA, mucosal IgA, CD4(+) T lymphocytes and B lymphocytes

of Dim-1 group were produced. While compared with the negative control group, increased numbers of blood eosinophils and lymphocytes and declined haemoglobin level were observed in the Dim-1 group after L3 challenge. The preliminary study suggest that recombinant H. contortus Dim-1 DNA vaccine induced partial immune response and has protective potential against goat haemonchosis. (c) 2013 Elsevier Ltd. All rights reserved.”
“In this work, we combined an experimental technique and a detailed data analysis to investigate the influence of an applied pressure on the anisotropic dielectric functions of highly oriented poly (p-phenylene vinylene) (PPV). The dielectric constants were derived from polarized reflectance spectra recorded through a diamond anvil cell up to 50 kbar. The presence of the diamond anvils strongly affects measured spectra requiring the development in an optical model able to take all spurious effects into account. A parametric procedure was then applied to derive the complex dielectric constants for both polarizations as a function of pressure. A detailed analysis of their pressure dependence allows addressing the role of intermolecular interactions and electron-phonon coupling in highly oriented PPV.