CPA showed significant reduction of collagen deposition in the abstinent group compared to the recent drinkers (1 4.2 % vs 25.6 %, p= 0.04).

Significant over-expression of MMP-9 was observed in the abstinent group as compared to the recent drinkers (2.0 vs 0.33, p=0.013). Immunostains for TIMP-1 &2 and MMP-2 were negative in both groups. Conclusions: This study demonstrates evidence of fibrosis regression in liver transplanted patients after a long period of alcohol abstinence. IHC study further supports the role of MMP-9 in this fibrosis regression. Comparison of immunohistoloical features of abstinent liver transplant patients and recent sobriety   M:F Age Steatosis (%) Mallory-Denk hyalines CPA (%) MMP-9 expression * p < 0.05, ns: not significant Disclosures: The following people have nothing to disclose: Hongfa Zhu, Xuchen Zhang, Fal-guni Parikh, HaiShan Wu, Neil D. Theise, Stephen see more C. Ward, Swan N. Thung, Thomas D. Schiano, M. Isabel Fiel Background/Aim: Cirrhosis is a long-term consequence of chronic hepatic injury with fibrosis and no

effective therapy except liver transplantation is currently available for decom-pensated cirrhosis. However, some practical limitations in liver transplantation lead us to a need for new therapeutic paradigm in this field. Recent reports have shown that the mesenchymal stem cells(MSCs) have the plasticity to differentiate into some kinds of tissue cells and improve organ function. Hence, STA-9090 price we investigated the effect of direct inoculation of human bone marrow derived MSCs(BM-MSCs) in thioacetamide(TAA)-induced cirrhosis in a rat model. Methods: Adult Sprague-Daw-ley rats were allocated into three groups (each group, n = 15) as follows: G1, shame; G2, TAA-control; G3, TAA+BM-MSC. To induce cirrhosis, 200mg/kg TAA injection was done twice a week for 12weeks in G2 and G3. 2×106 cells of amplified human BM-MSCs were injected directly into the right liver lobe twice, at weeks 6 and 8 in G3. At 12 weeks, the effect of BM-MSCs on cirrhosis was analyzed histomorphologically using

Laennec scores. α-Smooth muscle actin(α-SMA) expression by immunohistochemical staining, relative expression of collagen type 1, and transforming growth factor β (TGF-β) were also evaluated by real-time ifenprodil reverse transcriptase-polymerase chain reaction. Results: Laennec scores were 0, 5.4±0.7 and 3.7±1.06 in G1, G2 and G3, respectively. Histologically, BM-MSCs injected group (G3) showed significant suppression of hepatic fibrosis compared with TAA-control group (G2)(P < 0.001). Expressions of α-SMA(%) were significantly lower in G3 than in G2 (3.08±1 .26 vs. 7.00±4.12, P < 0.05). Also, the relative expression of collagen type 1 and TGF-β1 in RT-PCR were 0.64±0.24, 2.06±0.51, 1.32±0.31 and 0.62±0.28, 5.89±3.05, 2.22±1.41 in G1, G2 and G3, respectively P < 0.005).