It can also provide information on the taxonomic assignments of specific T-RFs without the need for comprehensive complementary Mocetinostat clone libraries. Availability and requirements Project name: T-RFPred Project home page: http://​nodens.​ceab.​csic.​es/​t-rfpred/​ Operating

systems: Linux (tested in Debian, Ubuntu and RHEL), Mac OS X (tested in MacOS X 10.5 and Mac OS X 10.6), Windows (via a Xubuntu VMware image) Programming language: Perl Other requirements: BioPerl, BLAST and EMBOSS License: none Any restrictions to use by non-academics: none Acknowledgements This work was supported by grant PIRENA CGL2009-13318-CO2-01/BOS to EOC, grant CTM2007-63753-C02-01/MAR to JMG, and grant CONSOLIDER-INGENIO2010 GRACCIE CSD2007-00067 to AFG from the Spanish Ministry of Science and Innovation, and grant OCE-0550485 from the National Science Foundation to AB. Electronic supplementary material Additional file 1: “”Project website”", “”Additional Experimental Procedure”" and “”Supplementary Tables

S1-S3″”. Project website. Webpage to download T-RFPred. Additional Experimental Procedure. Origin of chromatograms and reference datasets to label the peaks on Figure 2. Supplementary Tables S1-S3. Typical output of T-RFPred for the clone sequences from [4–6], respectively. (PDF 86 KB) References 1. Liu BMS202 W-T, Marsh TL, Cheng H, Forney LJ: Characterization of microbial diversity by (-)-p-Bromotetramisole Oxalate determining terminal restriction fragment length polymorphisms of genes encoding 16S rRNA. Appl Environ AZD3965 purchase Microbiol 1997, 63:4516–4522.PubMed 2. Marsh TL: Terminal restriction fragment length polymorphism (T-RFLP): an emerging method for characterizing diversity among homologous populations of amplification products. Curr Opin Microbiol 1999, 2:323–327.PubMedCrossRef 3. Blackwood CB,

Marsh T, Kim S-H, Paul EA: Terminal restriction fragment length polymorphism data analysis for quantitative comparison of microbial communities. Appl Environ Microbiol 2003, 69:926–932.PubMedCrossRef 4. González JM, Simó R, Massana R, Covert JS, Casamayor EO, Pedrós-Alió C, Moran MA: Bacterial community structure associated with a dimethylsulfoniopropionate-producing North Atlantic algal bloom. Appl Environ Microbiol 2000, 66:4237–4246.PubMedCrossRef 5. Mou X, Moran MA, Stepanauskas R, González JM, Hodson RE: Flow-cytometric cell sorting and subsequent molecular analyses for culture-independent identification of bacterioplankton involved in dimethylsulfoniopropionate transformations. Appl Environ Microbiol 2005, 71:1405–1416.PubMedCrossRef 6. Pinhassi J, Simó R, González JM, Vila M, Alonso-Sáez L, Kiene RP, Moran MA, Pedrós-Alió C: Dimethylsulfoniopropionate turnover is linked to the composition and dynamics of the bacterioplankton assemblage during a microcosm phytoplankton bloom. Appl Environ Microbiol 2005, 71:7650–7660.PubMedCrossRef 7.