Sun et al. [11] assessed the effects of SP on adipogenesis in mature adipocytes in vitro and the effects against obesity in vivo. As a result, an 8-week SP treatment period inhibited both preadipocyte differentiation and adipogenesis and reduced the body and fat weights in induced-obese rats that were fed a high-fat diet. Additionally, Lee et al. [22] reported that SP treatment reduced fat accumulation by up-regulating

leptin in 3 T3-L1 fibroblasts. We previously reported that SP treatment promoted resting fat oxidation [15]. To our knowledge, the results of the present Omipalisib study provide the first evidence of a further increase in fat oxidation during exercise in mice treated with SP relative to those not treated with SP. Taken together, these data indicate that SP might increase the exercise capacity by modulating fat metabolism during

exercise. The present study demonstrated no significant glycogen-saving effects of a 2-week SP treatment regimen during exercise. However, somewhat surprisingly, the glycogen concentration in the white gastrocnemius muscle tissue this website increased in the SP group during the recovery period (at 1 h post-exercise). Previous studies have reported that SP treatment for more than 1 month yielded glycogen-saving effects [12, 13]; however, these previous studies did not analyze ARN-509 ic50 the glycogen levels at the post-exercise recovery time point. The discrepancy between the current Chlormezanone and previous studies regarding the glycogen-saving effect might have been due to the SP treatment duration or dose or the different types of

exercise to which the animals were subjected. A number of investigators have reported post-exercise increases in the total glycogen synthase activity levels in skeletal muscle tissues [23–25]. Therefore, it appears that increase glycogen synthase activity would exert beneficial effects with SP at 1 h post-exercise. It remains unclear why the 2-week SP treatment used in the present study led to increased post-exercise accumulation. We also found that glucose, FFA and insulin levels in plasma did not differ between the groups. Particularly, the glucose level was significantly decreased at immediately after exercise and increased 1 h post-exercise in the SP group. However, the alteration of the glucose level in SP group seems to be involved with the glycogen synthase in the recovery period. In a future study it will be necessary for us to study the effect of SP on fat and carbohydrate metabolism related to gene expression in detail. We could not exclude the possibility that higher fat oxidation of SP mice would be due to lower intensity of exercise after 2-wk training but not to a direct effect of SP.